FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59942311
Sequences flagged as poor quality0
Sequence length101
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28908004.822636884987634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA203300.011.33141914-15
ATCGGAA460950.010.2156382
GAGCGTC511700.08.9823069
AGAGCGT518450.08.8497418
GATCGGA520400.08.8254791
TCGGAAG544350.08.7413193
CGGAAGA566000.08.562254
AAGAGCG622700.07.7056687
CCGGGAT144900.07.34262041
TAGGGAA238750.07.181795618-19
CGTGTAT55900.06.92509514-15
CGGGAAT105450.06.6655111
AGCGTCG365600.06.65037210-11
CTGGAAT221550.06.43201641
CCGGAAT70350.06.3642321
CCCGGAT99100.06.36392161
CGGAAAT74250.06.35414031
GGGGAAT296200.06.2250221
GGGAAAT235250.06.2006921
GGGAATC135300.06.15013932
Produced by FastQC (version 0.11.5)