FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n02_HBHm16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n02_HBHm16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32161670
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT100350.09.6325951
GCGGAAT56150.08.225961
GGGGAAT173550.07.9842291
GCGTCGT245100.07.954785394-95
CGGGGTT105650.07.79724551
CGGGCAT132750.07.6761581
AGAGCGT349550.07.656728794-95
CCGGGAT108400.07.6433651
GGGAAAT250700.07.54051161
CGCGAGT31650.07.52247761
TCGCACT57250.07.4800792
GATCGGA599350.07.44121294-95
CTCGGAT94400.07.414981
AGATCGG673950.07.33990194-95
CGGGAAT72400.07.300441
CGTCGTG239600.07.294904794-95
CCCGAAT73200.07.2857041
ATCGGAA564500.07.202254394-95
AGCGTCG294650.07.19736794-95
GCGGTAT31150.07.18463041
Produced by FastQC (version 0.11.5)