FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n02_HBHm15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n02_HBHm15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25999988
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAT101200.07.95078371
GGGAAAT199300.07.66834161
CGGCAAT65350.07.6497531
CGGGGAT104250.07.5811621
CGGGAAT68750.07.54844571
CGCGGAT50550.07.4406291
CCGGAAT60650.07.22205451
CTCGGAT78550.07.21281051
GGGGAAT146400.07.0895581
CGGGCAT123400.07.0605691
ATCGGAA221000.07.059990494-95
AATCCAA161100.07.04883055
AGATCGG289250.07.019784594-95
CGGTAAT29850.07.01797961
CGGGTAT38150.06.98871371
CGCGTAT9556.5899614E-56.9795661
GAATCGG45550.06.88677174
TCGGATT44850.06.78884272
CGCGAGT38600.06.78389451
GATCGGA241950.06.772585494-95
Produced by FastQC (version 0.11.5)