FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n02_HBHm14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n02_HBHm14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34580780
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG28350.010.73376753
CGCGTAT6959.308096E-79.5915221
GGGGGGA552550.08.1889322
CCGGGAT108750.08.18761
CAACGAT101950.08.1732571
AAAGGGG373100.08.08365780-81
CGCGAAT13551.382432E-108.082261
CTCGGAT108600.07.97968671
CGCTAAT16301.8189894E-127.8871571
CGCCAAT67750.07.8011391
GCGGAAT63550.07.7922361
CGGGGAT108850.07.78638551
GCGGGAT86900.07.671011
GGCGAAT79200.07.63524251
CGGGAAT74750.07.5164911
GCGTCGT267800.07.466722594-95
AACGATT80300.07.4657492
CGGGCAT136250.07.44367461
GATCGGA649100.07.361136494-95
GCGGTAT37700.07.3253931
Produced by FastQC (version 0.11.5)