FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n02_HBHm13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n02_HBHm13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31231226
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG334240.10702109484911032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAT101350.09.3490181
ACGATCG26000.08.4120923
CGGGGAT98000.08.4053681
CGATCGA26850.08.319914
GATCGGA465850.07.447924694-95
TAACCGA45350.07.436094816-17
CGGGATT57900.07.4011741
GATCGAA59250.07.3778585
GGGAAAT237000.07.37317281
GGGGAAT162800.07.31176571
GCCGATT36200.07.23420521
AGATCGG533200.07.21087394-95
CGATTCA54050.07.21079644
CGGGAAT70150.07.1947371
GCGGAAT59050.07.1764031
GTCGGAT37100.07.0587121
ATCGGAA434200.07.033674794-95
CGGGGTT106900.06.9929211
CGAAACG47550.06.99226226-27
CTCGGTT69550.06.98296261
Produced by FastQC (version 0.11.5)