FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n02_HBHm12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n02_HBHm12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34158637
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA610650.010.3792922
CTCGGGT99350.08.5799811
CCGGGAT113250.08.3678911
AAAAGGG442550.08.25320480-81
AAAGGGG431550.08.12789380-81
AAGGGGG452500.07.898528682-83
CGGGGAT111900.07.83048961
GCGGAAT64500.07.82613471
TCGCGTT34000.07.68330530-31
CCGGAAT72200.07.5191511
GGGAAAT282350.07.5053921
CTCGTAT33750.07.4783071
CCGGCAT137850.07.46187831
GGGAAAA454350.07.36989422-23
GCGTCGT287900.07.366118494-95
CGCGTAA11151.2542296E-67.26064541
AGAGCGT402500.07.233583594-95
TCGGGTC80950.07.2300972
AAAAAGG604850.07.20864478-79
CGAATCG23750.07.20755343
Produced by FastQC (version 0.11.5)