FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n02_HBHm11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n02_HBHm11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29509269
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAAT147550.08.5196071
CGCGAAT13501.4551915E-118.4651031
CGGGGAT94400.08.423631
CGAATCG21500.08.18271353
GGGAAAT231800.07.7648521
CCGGGAT102500.07.6650481
CGCGAGT32800.07.54891161
CTCGGAT87400.07.5183491
TCGCGTT31050.07.495482430-31
GATCGGA432650.07.415645694-95
TCGCACT56700.07.38485152
ATCGGAA406700.07.369117794-95
CCGGAAT63900.07.22812561
CGGGAAT67800.07.093271
CGGGCAT122050.07.02246481
AGATCGG492650.06.97525994-95
CTCGCAC87450.06.9151041
CGGGGTT102750.06.904931
CGACGAT39350.06.89739271
CCGGTAT39500.06.87121
Produced by FastQC (version 0.11.5)