FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n02_HBHm10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n02_HBHm10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14569488
Sequences flagged as poor quality0
Sequence length101
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA11201.7285129E-77.6334249
CCGGGAT70500.07.63172531
CCGGAAT34700.07.5468661
CTCGGAT46100.07.3331541
TACTCTA20500.07.18245179
CGGGCAT77800.07.16043951
GAAAAAT143550.06.99862431
CTGGAAT118350.06.8393381
GGGAAAT110200.06.826671
CGTATCC17606.9485395E-106.7541543
CTCGAAT26250.06.71128751
AATCCAT53150.06.7050475
CGCGTAT7850.00303319666.6720061
GATCCAA53500.06.6611825
CTTAGAT36100.06.59471751
CGGGAAC54350.06.4828441
ATCGAAC14703.3845026E-76.4632236
AACTCAC37950.06.38560725
GGGGAAT103300.06.3608061
GAATCTA31400.06.3581684
Produced by FastQC (version 0.11.5)