FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n02_HBHm09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n02_HBHm09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32575629
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAT76250.09.2412141
CCGGGAT108700.08.9790721
GTCGATT43800.08.696071
CGGGGAT99150.08.1632541
GCGGGAT84050.08.0437351
GGGAAAT256200.07.8608291
GGCGAAT42200.07.7847341
CGCGGGT69000.07.7281591
CGGCGAT62550.07.68778471
GGGGAAT165700.07.64304261
CGCGGAT45150.07.59244871
CGGCAAT67400.07.487781
CTCGGAT96400.07.30956941
CCGGAAT70600.07.28326561
GGAAAAT323200.07.23298031
CGGCATT74300.07.1715062
CTCGGGT97050.07.1624971
CGGGCAT130350.07.1589971
CGGGATT62650.07.0675521
CGCGTAT8801.8310636E-47.033441
Produced by FastQC (version 0.11.5)