FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n02_HBHm08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n02_HBHm08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34290368
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA587200.013.1427012
CGCGAAT13950.09.2163381
AAAAGGG449150.08.76643580-81
AAGGGGG441450.08.63960582-83
CGGGAAT81300.08.5512811
GGGAAAA473000.08.38978622-23
CGGGGAT105900.08.3184921
CGGGGTT111350.08.2962221
CTCGGAT100450.08.0587531
GGGGGAG637300.08.0577953
CCGGGAT115550.07.99467131
CGCGGAT48800.07.90376571
GCGTCGT320750.07.744538394-95
TCGCACT64300.07.54760272
GATCGGA779900.07.51513494-95
CTCGGTT74150.07.5135221
TAAAAAA956600.07.49731670-71
AGAGCGT452100.07.4695794-95
CTCGGGT102350.07.4439081
TCGGGTC80300.07.34726952
Produced by FastQC (version 0.11.5)