FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n02_HBHm07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n02_HBHm07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32708215
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA552050.011.755452
CGGGGAT104300.08.9927511
CGCGAAT13900.08.9057241
CGCGATT11503.8744474E-108.694251
CTCGGAT98700.08.6829241
CGGGAAT74650.08.5464481
GGCGAAT43000.08.4150321
CGCGGAT50600.08.3743321
CCGGGAT104100.08.09530451
CGGGTAT36450.07.96788361
AAAAGGG404400.07.916043880-81
GCGTCGT277750.07.874736394-95
GGGAAAA422650.07.74858522-23
GCGGGAT81400.07.72076231
AAGGGGG401750.07.714066582-83
GGGGGAG601100.07.58540343
AATCGTA29750.07.50648555
GCGGTAT32000.07.43927671
CCGGAAT66200.07.40781161
GTCGTGT251950.07.33323694-95
Produced by FastQC (version 0.11.5)