FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n02_HBHm06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n02_HBHm06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34054653
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA522550.014.6327432
CGCGTAT8752.5713234E-89.2515011
GGGAAAA459650.08.74714122-23
CGCGAAT14700.08.7461671
AAGGGGG397600.08.43962282-83
AAAAGGG416550.08.38063180-81
GGGGGAG572500.08.2726223
CGGGAAT78800.07.97662261
CCGGGAT114200.07.96413471
TCGCACT65200.07.9545692
CGGGGAT102300.07.81996771
CCGGAAT73250.07.80090331
GCGTCGT296500.07.721140494-95
GCGGGAT82600.07.6096591
AGGGGGG529300.07.600445384-85
GTCCGAT29200.07.50146871
CGGGATT61050.07.4098471
CGCGGGT69250.07.2888231
GATCGGA696800.07.218499794-95
CTCGGAT99100.07.2075711
Produced by FastQC (version 0.11.5)