FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n02_HBHm05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n02_HBHm05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33661810
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA561400.012.2894552
CGGGGAT113350.08.6539761
CCGGGAT112800.08.6539571
CGCGAAT12151.2551027E-108.6221671
AAAAGGG426750.08.35845680-81
GGGAAAA453350.08.14575422-23
AAGGGGG424150.08.11853982-83
CGGGCAT130850.07.7877151
CGCGGGT70400.07.7784881
GCGTCGT274150.07.76157494-95
CGGGGTT111850.07.7057081
GCGGGAT87700.07.60148531
GGGGAAT188300.07.5359141
GATCGGA646450.07.494199394-95
CGCGAGT38900.07.46707061
AAAGGGG411450.07.462960780-81
CGGGAAT72450.07.2954921
CGTCGTG267350.07.26612994-95
GGGAAAT268400.07.25622561
ATCGGAA617900.07.252432394-95
Produced by FastQC (version 0.11.5)