FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n02_HBHm04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n02_HBHm04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34210950
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA612900.012.0488532
GGCGAAT45900.09.0255551
AAAAGGG466600.08.8864880-81
AAGGGGG470300.08.49840682-83
GGGAAAA479650.08.42691422-23
GCGGTAT32800.08.2750071
CGCAAAT52800.08.0264491
CTCGGAT98150.07.95647861
CGCGTAT7201.402813E-47.9362641
CGGGTAT37950.07.9048961
CGGGGAT101350.07.84621241
CGGGAAT78100.07.8041621
CCGGGAT110550.07.7962751
CGCGGAT49200.07.7426971
CGCGGGT65650.07.68844461
GGGGGAG637850.07.64143473
CGCGATT12551.798253E-87.58845951
GATCGGA585000.07.45731194-95
CGCGAGT36300.07.34596351
GGGAAAT284950.07.30264431
Produced by FastQC (version 0.11.5)