FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n02_HBHm03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n02_HBHm03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31809191
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA486250.012.3391322
CGCGAAT11705.456968E-129.360791
CGGGAAT72150.09.1077961
CGGGGAT92250.08.7235021
CGCGAGT33200.08.3187951
CCGGAAT70800.08.0708361
CCGGGAT104000.08.0584191
CGGGCAT129950.07.95158961
GGGAAAT253000.07.8108471
GCGGTAT31800.07.786581
AAGGGGG360850.07.54855482-83
CTCGGAT97800.07.54680871
GCGGGAT77700.07.53797441
GCGTCGT233800.07.496208794-95
AAAAGGG377500.07.454682480-81
GGGAAAA408800.07.38361222-23
GCGGGTT51950.07.33288671
CGGGATT54800.07.29909851
ATCGGAA513550.07.283290494-95
GGGGGAG543900.07.2122723
Produced by FastQC (version 0.11.5)