FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n02_HBHm02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n02_HBHm02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32348479
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAT107350.08.5598371
CGCGAAT15450.08.3204261
CGGGGAT108150.08.3204261
GGGGAAT168700.08.2409641
CTCGGTT71700.08.2340361
CGGGGTT110450.08.1040551
CGCGGAT48850.08.089541
CGGGCAT132450.07.8723141
CGGGAAT77150.07.8375091
CTCGGAT96750.07.57844351
GGGAAAT253250.07.57645131
CGGAAAT72200.07.38568971
CGCGGGT69750.07.30381631
AATCGTG61450.07.1135585
CGGCAAT68700.07.06893061
CGGGTAT40100.07.00515751
CTGGAAT309600.06.98176341
ATCGGAA322450.06.952495694-95
GATCGGA339850.06.92496494-95
GTCGATT45000.06.87719151
Produced by FastQC (version 0.11.5)