FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n02_HBHm01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n02_HBHm01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31203920
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA521850.013.3920892
CGCGTAT6908.444622E-79.6601931
CCGGGAT99350.08.7218871
AAGGGGG381600.08.60064782-83
AAAAGGG395200.08.57509380-81
GGGAAAA435250.08.43546722-23
CGGGAAT69250.08.3190261
GGGGGAG548650.08.119413
GGCGAAT39850.07.7659021
GGGAAAT246200.07.7159921
CGCGAAT12351.3389581E-87.71027661
CGCGAGT32300.07.66492131
CGGGGAT92300.07.5826771
GTCGATT41000.07.5480781
CCGGAAT64600.07.5175191
CTCGGAT90950.07.32878971
CGGGATT56600.07.3182921
CGCTAAT15001.1823431E-97.30034641
CGGAAAT67350.07.281261
GGGGAGA434150.07.22486544
Produced by FastQC (version 0.11.5)