FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59942311
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7034511.17354667890599No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT1962030.32731971244819036TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1484030.2475763738905562TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT1359610.22681975007603558TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC2437450.054.263328
GAGCACA2534800.052.1781439
CGGAAGA2642200.050.2903064
TCGGAAG2681550.049.5219963
ATCGGAA2726050.048.5970762
AAGAGCA2788600.047.7220887
GATCGGA2751350.046.433731
GAAGAGC2929600.045.3034176
GGAAGAG3184750.041.9273725
GAATCGG719250.041.48481840-41
AATCGGG704500.041.29504840-41
CGGAGAA1181250.038.58156636-37
CCGGAGA1212050.038.15800534-35
TCCGGAG1234200.037.7290634-35
CTCCGGA1249800.037.49932532-33
GTCACTC1281000.037.0628828-29
TACTCGA313400.036.94962336-37
AGAATCG962100.036.8437238-39
CACTCCG1274600.036.60622830-31
CTCGTAT290750.036.4082344-45
Produced by FastQC (version 0.11.5)