FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n01_HBHm16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n01_HBHm16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32161670
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT619330.1925677366878026TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT423980.1318277315823463TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGG232550.037.5825440-41
GAATCGG240700.037.29669640-41
CACTCCG378250.034.5796730-31
AATCGCG32650.033.96943740-41
GAGCACA791850.033.6291929
AGAATCG311450.033.2561838-39
AGAGCAC807800.032.823958
ACTCCGG400500.032.66440632-33
ATCGGAA855700.031.0078872
CGGAGAA416100.030.93721436-37
TCGGAAG867650.030.6689363
CGGAAGA871950.030.5176934
CCGGAGA430250.030.36151534-35
TCCGGAG436350.030.06770934-35
GATCGGA885250.029.613421
GTCACTC448400.029.46670228-29
CTCCGGA447600.029.4288432-33
ATCGGGG246100.028.45894842-43
AAGAGCA945200.028.1930477
CGTCTGA478900.027.88230316-17
Produced by FastQC (version 0.11.5)