FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n01_HBHm15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n01_HBHm15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25999988
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGG73100.022.67759340-41
GAATCGG79400.021.3269140-41
CACTCCG125100.018.68169630-31
AGAATCG108750.017.66808338-39
ACTCCGG145250.016.15536732-33
CCGGAGA166150.014.22315134-35
CGTCTGA170900.014.20281516-17
GTCACTC170350.014.19347528-29
ACACGTC168650.014.11043112-13
CGGAGAA166900.013.97429536-37
ATCGGAA342500.013.9379392
ACGTCTG173000.013.79676214-15
GAGCACA375250.013.3801689
AGAGCAC373350.013.2446668
TCCGGAG180400.013.17864234-35
TCGGAAG366300.013.1621623
CACGTCT185250.013.11519214-15
GATCGGA355900.013.0661791
CTCCGGA183000.013.03042832-33
AATCGCG12950.013.02109440-41
Produced by FastQC (version 0.11.5)