FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n01_HBHm14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n01_HBHm14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34580780
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT747070.2160361912021649TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT519340.15018169052288582TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG294300.037.4765940-41
AATCGGG286750.037.2623940-41
GAGCACA881800.036.2262089
AGAGCAC901500.035.403018
CACTCCG448050.034.98144530-31
AGAATCG358300.034.9792938-39
TCGGAAG958650.033.203223
ATCGGAA959600.032.9916732
ACTCCGG478550.032.90044432-33
CGGAAGA970050.032.852244
CGGAGAA477250.032.41230436-37
GATCGGA978900.031.5016981
CCGGAGA504150.031.0455834-35
GTCACTC512500.030.84658628-29
TCCGGAG513700.030.73195834-35
AATCGCG42550.030.64297540-41
CTCCGGA520100.030.4182132-33
AAGAGCA1060400.030.223247
ATCGGGG287900.029.74693142-43
CGTCTGA567650.028.24213816-17
Produced by FastQC (version 0.11.5)