FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n01_HBHm13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n01_HBHm13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31231226
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT392830.12578116529911443TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGG165650.032.53098340-41
GAATCGG172300.032.26786440-41
CACTCCG255300.029.93763430-31
AGAATCG209600.029.77899738-39
ACTCCGG281100.027.1645232-33
GAGCACA619000.025.3469099
CGGAGAA304150.024.91059336-37
AATCGCG23550.024.80837240-41
CCGGAGA308850.024.79297434-35
AGAGCAC634300.024.623188
TCCGGAG320200.024.04766334-35
GTCACTC327700.023.72952328-29
ATCGGGG191400.023.67510642-43
ATCGGAA652400.023.6257842
CTCCGGA329850.023.40897632-33
TCGGAAG662950.023.3361993
CGGAAGA680150.022.7741074
GATCGGA678000.022.4606381
CGTCTGA351250.022.20602416-17
ACGTCTG354150.021.91551414-15
Produced by FastQC (version 0.11.5)