FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n01_HBHm12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n01_HBHm12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34158637
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT839910.24588510367085198TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT557750.1632822761634195TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGG305050.038.53024740-41
GAATCGG316300.038.05333740-41
GAGCACA942650.037.56179
AGAGCAC962550.036.7111178
CACTCCG481350.036.03975730-31
AGAATCG388950.035.7036938-39
AATCGCG43850.034.66296440-41
ATCGGAA1017800.034.5208172
ACTCCGG506400.034.2618232-33
TCGGAAG1028600.034.260553
CGGAAGA1035000.034.030544
CGGAGAA519450.032.90266436-37
GATCGGA1050400.032.8390581
CCGGAGA535700.032.2193834-35
TCCGGAG544200.031.89507534-35
CTCCGGA547700.031.75632532-33
AAGAGCA1120950.031.5995167
GTCACTC557850.031.44698328-29
ATCGGGG305050.030.8148142-43
TCACTCC600700.029.09666630-31
Produced by FastQC (version 0.11.5)