FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n01_HBHm11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n01_HBHm11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29509269
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT431960.14638112519832328TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT303220.10275415497415406TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGG174300.034.88178340-41
GAATCGG181250.034.52698540-41
CACTCCG282700.031.51375630-31
AGAATCG233600.030.39999838-39
AATCGCG23650.030.02590640-41
ACTCCGG305650.029.16310932-33
GAGCACA631250.029.0764669
AGAGCAC634800.028.651968
ATCGGAA645800.027.9641972
TCGGAAG656250.027.577413
CGGAGAA318500.027.5466636-37
CGGAAGA668050.027.1259464
CCGGAGA330950.026.97695234-35
GATCGGA665100.026.6742341
TCCGGAG334900.026.63749334-35
GTCACTC341000.026.37688428-29
CTCCGGA342800.026.07884832-33
ATCGGGG188700.025.96477142-43
CGTCTGA365200.024.96059416-17
ACGTCTG369000.024.58631514-15
Produced by FastQC (version 0.11.5)