FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n01_HBHm10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n01_HBHm10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14569488
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT5801.1392776E-59.8276621
CGGGAAT34100.08.4971331
GTCGATT17950.08.4680321
GGGGAAT74600.08.3412181
CGAAAAT25950.08.2370591
GTCGTTT15001.8189894E-128.23339751
GCGGTAT16500.08.0606681
GGGAAAT123150.07.8685141
GCCGATT16653.6379788E-127.70276261
CGGCAAC35500.07.62681961
CGGCGAT28050.07.62038041
TGCGCGA22100.07.5227349
GTCGGAT17207.2759576E-127.4564531
GCGGATT26800.07.4440881
GTCGGTT25550.07.43645671
GTCGCAT14708.112693E-107.43203071
TAGGTCG10303.0982737E-67.37877373
AATCGAT15804.8567017E-107.21546845
AATCGAG25750.07.1944535
AATCCGA23250.07.15081365
Produced by FastQC (version 0.11.5)