FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n01_HBHm09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n01_HBHm09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32575629
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT494010.15165017995508237TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT338480.10390589848625793TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGG193950.034.7030940-41
GAATCGG200700.034.5654640-41
AGAATCG250350.031.17423238-39
CACTCCG309700.030.73753230-31
AATCGCG23150.029.75124540-41
ACTCCGG338400.028.21481332-33
CGGAGAA348450.027.0942336-37
GAGCACA734950.026.699249
CCGGAGA357300.026.6756934-35
AGAGCAC747950.026.031968
TCCGGAG371250.025.76289634-35
CTCCGGA374450.025.49844732-33
GTCACTC377600.025.48099528-29
ATCGGGG221650.025.39450542-43
ATCGGAA786050.024.449342
TCGGAAG795500.024.207083
CGGAAGA804000.023.9749374
GATCGGA808400.023.2681061
TCACTCC422800.022.8353730-31
CGTCTGA430150.022.6051716-17
Produced by FastQC (version 0.11.5)