FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n01_HBHm08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n01_HBHm08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34290368
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT2997970.8742892464729454TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG421500.043.6563944-45
CGTATGC433300.043.13619246-47
TATGCCG435400.042.87911248-49
CTCGTAT397850.042.50866344-45
TACTCGA451150.041.09993736-37
TTACTCG469400.040.1042334-35
ACTCGAT442200.039.5416736-37
TCTCGTA414100.038.8389842-43
ATGCCGT490550.037.72437748-49
GAGCACA1028200.037.4060759
GTATGCC499400.037.27931246-47
AGAGCAC1047250.036.6530158
CCGTCTT503700.036.4754252-53
ATTACTC524600.036.35972234-35
GCCGTCT507600.035.90022350-51
ACATTAC542900.035.42308432-33
CTCGATC457350.035.22971338-39
TGCCGTC530400.035.13611250-51
ATCTCGT463000.034.3830442-43
TCGATCT464350.034.07462738-39
Produced by FastQC (version 0.11.5)