FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n01_HBHm07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n01_HBHm07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32708215
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT2606260.7968212267162852TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG370850.043.31083744-45
CGTATGC379950.042.998546-47
TATGCCG380150.042.75725648-49
CTCGTAT349050.042.2123644-45
TACTCGA395500.040.7038136-37
TTACTCG409950.039.92375634-35
ACTCGAT390200.038.97411736-37
TCTCGTA363050.038.4520942-43
ATGCCGT436200.036.9854448-49
GTATGCC442450.036.74207746-47
GAGCACA913400.036.715589
AGAGCAC923850.036.233278
ATTACTC464650.035.6122934-35
CCGTCTT449800.035.40779552-53
GCCGTCT455800.034.92590750-51
CTCGATC400900.034.8171638-39
ACATTAC480050.034.7963632-33
TGCCGTC475600.033.9912250-51
ATCGGAA980900.033.9552232
ATCTCGT409000.033.8243542-43
Produced by FastQC (version 0.11.5)