FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n01_HBHm06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n01_HBHm06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34054653
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT2797840.8215734865952092TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG390650.043.46845644-45
CGTATGC399300.043.19289446-47
TATGCCG403250.042.67563248-49
CTCGTAT372550.041.95940444-45
TACTCGA421900.040.46460336-37
TTACTCG434300.040.08056634-35
ACTCGAT411000.039.15112336-37
TCTCGTA389750.038.0419642-43
GTATGCC462300.037.16293346-47
GAGCACA962700.037.055519
ATGCCGT464600.036.93300648-49
AGAGCAC976600.036.552428
CCGTCTT472450.036.0729552-53
ATTACTC491450.035.7241234-35
GCCGTCT480350.035.4103850-51
CTCGATC422050.034.93484538-39
ACATTAC512000.034.55955532-33
TGCCGTC503200.034.2696750-51
ATCGGAA1035400.034.200352
TCGGAAG1045150.033.9864853
Produced by FastQC (version 0.11.5)