FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n01_HBHm05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n01_HBHm05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33661810
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT2710710.8052775534054764TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG385350.043.5915544-45
CGTATGC395950.043.1563946-47
CTCGTAT363350.042.4529644-45
TATGCCG405200.041.95440748-49
TACTCGA414300.040.96037336-37
TTACTCG426450.040.45612734-35
ACTCGAT403250.039.5619436-37
TCTCGTA380900.038.4518642-43
GAGCACA928400.038.015559
AGAGCAC939800.037.4533238
GTATGCC455600.037.3444946-47
ATGCCGT456550.037.0015448-49
ATTACTC485250.035.93564634-35
GCCGTCT471600.035.51339750-51
TCGGAAG986650.035.4672363
ATCGGAA984400.035.456152
CCGTCTT475600.035.31461352-53
CGGAAGA998800.035.0977754
ACATTAC502850.034.98530632-33
CTCGATC418750.034.8468838-39
Produced by FastQC (version 0.11.5)