FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n01_HBHm04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n01_HBHm04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34210950
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT3043120.889516368297285TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG415100.043.70582644-45
TATGCCG425650.043.1917348-49
CGTATGC428100.043.07205646-47
CTCGTAT394350.042.13913344-45
TACTCGA442900.040.98109436-37
GAGCACA925350.040.7846689
AGAGCAC935100.040.2932628
TTACTCG457900.040.2557334-35
ACTCGAT431550.039.6593336-37
ATCGGAA959800.039.0959632
TCGGAAG968250.038.8488273
TCTCGTA410350.038.35473642-43
CGGAAGA987700.038.035944
ATGCCGT484600.037.6827648-49
GATCGGA977200.037.6417661
GTATGCC494450.037.090546-47
CCGTCTT495250.036.4838252-53
ATTACTC515050.036.22241634-35
GCCGTCT502000.035.7376250-51
CTCGATC441950.035.52790538-39
Produced by FastQC (version 0.11.5)