FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n01_HBHm03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n01_HBHm03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31809191
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT2310700.7264252649493663TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG342000.043.2908444-45
CGTATGC350350.043.0995546-47
TATGCCG356450.042.22215748-49
CTCGTAT323050.041.97064244-45
TACTCGA366850.040.626136-37
TTACTCG382450.039.82613434-35
ACTCGAT356750.039.03334836-37
TCTCGTA334750.038.16966642-43
GAGCACA830250.037.5835279
AGAGCAC833300.037.360348
ATGCCGT406200.036.75859548-49
ATCGGAA842600.036.6364862
GTATGCC414400.036.22599446-47
TCGGAAG857100.036.1943133
CGGAAGA867200.035.773054
ATTACTC437200.035.2244734-35
CCGTCTT421400.035.12833852-53
GATCGGA859600.035.1217541
ACATTAC448000.034.60346632-33
GCCGTCT427150.034.58304650-51
Produced by FastQC (version 0.11.5)