FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n01_HBHm02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n01_HBHm02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32348479
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1419670.4388676203292278TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG208950.041.57731644-45
CGTATGC216000.040.53910446-47
TATGCCG217100.040.09309848-49
CTCGTAT205200.038.91127444-45
TACTCGA228150.038.06966836-37
TTACTCG238800.036.91888434-35
ACTCGAT231950.035.84861436-37
TCTCGTA228600.033.6400142-43
ATGCCGT272700.032.0492748-49
ATCGGAA563850.031.9361382
GTATGCC281250.031.2015946-47
TCGGAAG586750.030.7467653
GATCGGA574200.030.6905211
GAGCACA603400.030.3947169
AGAGCAC602900.030.3332568
CCGTCTT289150.029.88094352-53
ATTACTC298950.029.71310234-35
CGGAAGA610600.029.6626244
CTCGATC258000.029.62349138-39
GCCGTCT292950.029.59864250-51
Produced by FastQC (version 0.11.5)