FastQCFastQC Report
Fri 7 Jun 2019
HHNYHBGXB_n01_HBHm01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNYHBGXB_n01_HBHm01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31203920
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT2667220.8547708108468423TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG370350.044.0171344-45
CGTATGC380450.043.41665346-47
TATGCCG382750.043.00697748-49
CTCGTAT353450.042.311944-45
GAGCACA818850.041.830999
ATCGGAA813400.041.8293462
AGAGCAC817500.041.7721028
TACTCGA393750.041.5839736-37
TCGGAAG824500.041.2899783
TTACTCG409100.040.67968434-35
GATCGGA821600.040.3078231
CGGAAGA849800.040.0664944
ACTCGAT389350.040.02868336-37
TCTCGTA365300.039.10600742-43
GTATGCC439650.037.44084546-47
ATGCCGT438100.037.38911448-49
ATTACTC458950.036.56648334-35
CCGTCTT445300.036.42181452-53
AAGAGCA940950.036.311687
ACATTAC472500.035.76418732-33
Produced by FastQC (version 0.11.5)