FastQCFastQC Report
Thu 30 Mar 2017
HHNK2BCXY_l02n03.33300000083bf0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNK2BCXY_l02n03.33300000083bf0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168180142
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT13419350.078.318671
AGCAGTG15226600.068.612572
GCAGTGG21553850.048.444233
CAGTGGT21884750.047.6150174
AGTGGTA22384350.046.4935235
GTGGTAT25973300.040.071726
TGGTATC26461050.039.3089337
GGTATCA27203450.038.276118
GTATCAA27560100.037.9780969
AGTCGTA154900.025.284565
CAACGCA27419600.021.72156712-13
ATCAACG27535300.021.6668410-11
GTCGTAT184050.021.3950316
TACATGG27104900.021.32980522-23
ACGCAGA27921850.021.29461114-15
AGAGTAC27891450.021.13545218-19
GCAGAGT27972300.021.09144616-17
AGTACAT27936700.020.98083120-21
CATGGGG16109450.020.36777724-25
CGTATCA199900.019.7412648
Produced by FastQC (version 0.11.2)