FastQCFastQC Report
Thu 30 Mar 2017
HHNK2BCXY_l02n01.33100000083bf6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNK2BCXY_l02n01.33100000083bf6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168180142
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTACC614050.018.1154731
GACTAAC510150.018.0045431
ACGAGCC717350.017.8467061
CGCTTCA444150.017.8426281
CGCTATC521600.017.7790011
TGATTTC487950.017.7288591
TGACGGC604200.017.7045061
CCTTACG461500.017.537151
TTTATGC431450.017.5228351
TATTACC576600.017.519471
TATGCCC533350.017.5019051
CCCATAC563000.017.4635561
CTTAGGA471000.017.4384611
GGATTAC497100.017.4110051
TGCCAAA601150.017.33511
GTACTTT704350.017.3248881
CGTCTAC420250.017.2953131
GGGCATC468400.017.1955761
AGTGTAC558650.017.1803538
TTTACTG350050.017.1245351