FastQCFastQC Report
Thu 30 Mar 2017
HHNK2BCXY_l01n03.33300000083c0e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNK2BCXY_l01n03.33300000083c0e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170125594
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT12283700.078.232021
AGCAGTG13918700.068.598742
GCAGTGG20301050.047.0361373
CAGTGGT20484100.046.519574
AGTGGTA21007600.045.267715
GTGGTAT24161650.039.363136
TGGTATC24606950.038.6571547
GGTATCA25216300.037.774318
GTATCAA25599350.037.4250879
AGTCGTA151150.024.2303855
CAACGCA25349900.021.5987312-13
ATCAACG25456750.021.57374210-11
TACATGG24855000.021.15039622-23
ACGCAGA25872600.021.09688814-15
AGAGTAC25723250.020.97473318-19
GCAGAGT25807400.020.92627716-17
AGTACAT25708750.020.7671420-21
GTCGTAT184400.020.3993516
CATGGGG14523500.020.18536624-25
CGTATCA187200.020.0913378
Produced by FastQC (version 0.11.2)