FastQCFastQC Report
Thu 30 Mar 2017
HHNK2BCXY_l01n01.33100000083c04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNK2BCXY_l01n01.33100000083c04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170125594
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC661150.018.3172531
GATGCTA625000.018.2869321
CCTACCA677050.018.0334721
ACTGAAC639450.017.9429441
AGCTTGA536800.017.8110921
TTGAACG533000.017.7659741
CATATTC529100.017.7554441
CCGGTAG530050.017.5445961
ACTGATG443300.017.5220341
AAACCTG608950.017.4218481
GGGCATC493150.017.4099271
CGTTGGG553100.017.3967591
AGACGTT710350.017.3855061
GGTATTG631150.017.2985271
GCAGTTA792900.017.2828871
GGGAATG594950.017.2593921
AACTCCC557500.017.1762431
CGCTATC459350.017.1760621
TCCCGAT682300.017.1715091
TTAACTC612500.017.1263521
Produced by FastQC (version 0.11.2)