FastQCFastQC Report
Tue 26 Nov 2024
HHNHYAFX7_n01_13016red.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNHYAFX7_n01_13016red.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8601825
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC861361.001368895554141TruSeq Adapter, Index 12 (100% over 50bp)
CCTCGCTTCCGGCAATACTCGTAAACCATATCAACCAGCTCGCTGACGTT256290.29794840048478083No Hit
CCTGCTTTTCGGGAAAACGGGATCACCACGATGGAACAGGTTAACGCAGG254010.2952978001761254No Hit
CCTAGGGCCTCGCAGACCCATCACGGGTCCAGATCCCGGTCTTTTCGCAG147710.17171937350504107No Hit
CCTCTGCAGTCGAGCCAGAACCCTGTTTGAATTCACTTCCGGCGTGAATG134830.15674580684912792No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC123220.14324867106689568TruSeq Adapter, Index 12 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)