FastQCFastQC Report
Tue 26 Nov 2024
HHNHYAFX7_n01_13015spRed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNHYAFX7_n01_13015spRed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6131824
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC219474235.79264505961032TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGTATGC605410.9873244894178306TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGGATGC468920.764731668749788TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATTC468900.7646990520275859TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGG326990.5332670996427816TruSeq Adapter, Index 6 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTTTGC268390.4377001035907097TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTAAGC233380.38060453137598205TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTGTGC154450.25188263720550363TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGGATGC138680.22616435174916955TruSeq Adapter, Index 6 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTAGTATGC130270.21244902006319816TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGATCTCGTATGC126760.2067247853167345TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATATCGTATGC111380.18164252594334082TruSeq Adapter, Index 6 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC86110.14043129744102245TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTGTGG79080.128966519586994TruSeq Adapter, Index 6 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAATATCTCGTATGC75850.12369891895135934TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGGATGG71550.11668632367791378TruSeq Adapter, Index 6 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGTATGG70900.11562628020634644TruSeq Adapter, Index 6 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGCCAATATCTCGTATGC65020.10603696387893717TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGCCAATATCTCGTATGC63840.1041125772690149TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAATATCTCGTATGC61800.10078567160440352TruSeq Adapter, Index 6 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)