FastQCFastQC Report
Tue 26 Nov 2024
HHNHYAFX7_n01_13015spIn.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNHYAFX7_n01_13015spIn.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17501934
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC1010590.5774161872625049TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC951310.5435456447270341TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATGC593970.3393739229047487TruSeq Adapter, Index 5 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGGATGC461610.263747994935874TruSeq Adapter, Index 5 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC255990.14626383575666552TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGGATGC223540.1277230276379742TruSeq Adapter, Index 5 (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)