FastQCFastQC Report
Tue 26 Nov 2024
HHNHYAFX7_n01_13014spRed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNHYAFX7_n01_13014spRed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14166452
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT3629192.561819995578286TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCGCGTAT488760.3450122867744161TruSeq Adapter, Index 27 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTGT300480.21210674345277136TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGGAT219330.15482352250231743TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCGCGTGT178760.12618544149233696TruSeq Adapter, Index 27 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCGGGTGT142940.10090035246651738TruSeq Adapter, Index 27 (97% over 45bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)