FastQCFastQC Report
Tue 26 Nov 2024
HHNHYAFX7_n01_13014in.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNHYAFX7_n01_13014in.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318634
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGGGCCTCGCAGACCCATCACGGGTCCAGATCCCGGTCTTTTCGCAG38941.2220918043899898No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGTGG36581.14802563442696TruSeq Adapter, Index 10 (97% over 42bp)
CCTCTGCAGTCGAGCCAGAACCCTGTTTGAATTCACTTCCGGCGTGAATG35851.1251153360909383No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC34101.0701933880251324TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGATGC32661.0250004707595548TruSeq Adapter, Index 10 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC27550.8646283824074017TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGATGC25530.8012327623543TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGATGG19120.6000615125818337TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGGATGC13880.43560950808764914TruSeq Adapter, Index 10 (98% over 50bp)
CCTCGCTTCCGGCAATACTCGTAAACCATATCAACCAGCTCGCTGACGTT13590.42650815669388703No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGTGC12270.3850813158671077TruSeq Adapter, Index 10 (97% over 42bp)
CCTGCCATCCAGTTCCCGCGAAGCTGGTCTTCAGCCGCCCAGGTCTTTTT11640.3653094145634176No Hit
CCTGCTTTTCGGGAAAACGGGATCACCACGATGGAACAGGTTAACGCAGG11380.357149582279355No Hit
CCTGGGATTTGTGGAAGGCGGAGAGTCAGTTCGCGGTACTGGAGGAGGCG11030.34616519266619383No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTATGC9480.29752003866505145TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTTGG8780.27555125943872905TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTTGC7420.23286905979901704TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGATGG7390.23192754068931754TruSeq Adapter, Index 10 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGGGG5950.1867346234237401TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGGATGC5640.17700559262351162TruSeq Adapter, Index 10 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGTATGC5370.16853192063621586TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGGTGC4540.1424832252678622TruSeq Adapter, Index 10 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGTTGC4500.1412278664549295TruSeq Adapter, Index 10 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGGTGG4030.1264774004029702TruSeq Adapter, Index 10 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTATGG3700.11612069019627536TruSeq Adapter, Index 10 (97% over 42bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3360.10545014028634735No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATTC3340.104822460879881TruSeq Adapter, Index 10 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)