FastQCFastQC Report
Tue 26 Nov 2024
HHNHYAFX7_n01_13013spRed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNHYAFX7_n01_13013spRed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5573403
Sequences flagged as poor quality0
Sequence length151
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC217467039.01871083070792TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC356850.6402730970647555TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAGCTCGTATGC148890.26714378988922927TruSeq Adapter, Index 9 (98% over 50bp)
CCTAGGGCCTCGCAGACCCATCACGGGTCCAGATCCCGGTCTTTTCGCAG134530.24137856171534697No Hit
CCTGCTTTTCGGGAAAACGGGATCACCACGATGGAACAGGTTAACGCAGG132070.23696474129001618No Hit
CCTCTGCAGTCGAGCCAGAACCCTGTTTGAATTCACTTCCGGCGTGAATG128970.23140260985972128No Hit
CCTCGCTTCCGGCAATACTCGTAAACCATATCAACCAGCTCGCTGACGTT127780.22926746908486612No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGCTCTCGTATGC119950.2152186016335083TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCGCGTATGC99970.1793697674472849TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCAGATCTCGTATGC93870.1684249281812207TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCAGATCTCGTATGC89720.16097884900840653TruSeq Adapter, Index 9 (98% over 50bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC71690.12862877491543317TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGGATGC57290.10279177730374064TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)