FastQCFastQC Report
Tue 26 Nov 2024
HHNHYAFX7_n01_13013spIn.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNHYAFX7_n01_13013spIn.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650426
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTGG252811.5317863387998008TruSeq Adapter, Index 8 (97% over 42bp)
CCTAGGGCCTCGCAGACCCATCACGGGTCCAGATCCCGGTCTTTTCGCAG170481.0329454334820223No Hit
CCTCTGCAGTCGAGCCAGAACCCTGTTTGAATTCACTTCCGGCGTGAATG161080.9759904412557727No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGATGC131000.7937344661317745TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGATGC113670.6887312730167847TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGATGG87990.5331350814880522TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTGC87750.531680911473765TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC75270.4560640707308295TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGGGG70130.42492059625817824TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC67830.41098480028792567TruSeq Adapter, Index 8 (98% over 50bp)
CCTCGCTTCCGGCAATACTCGTAAACCATATCAACCAGCTCGCTGACGTT59080.3579681851837041No Hit
CCTGCTTTTCGGGAAAACGGGATCACCACGATGGAACAGGTTAACGCAGG52850.3202203552294983No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGTTGG52280.3167667014455662TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATGC48660.29483297039673395TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGTTGC40210.24363406781037136TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGATGG37600.2278199689049979TruSeq Adapter, Index 8 (97% over 45bp)
CCTGCCATCCAGTTCCCGCGAAGCTGGTCTTCAGCCGCCCAGGTCTTTTT33400.20237199365497152No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGGTGC29990.18171066136864059TruSeq Adapter, Index 8 (97% over 45bp)
CCTGGGATTTGTGGAAGGCGGAGAGTCAGTTCGCGGTACTGGAGGAGGCG28980.17559102922518185No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGTATGC26820.1625034990965969TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGGTGG25220.15280903233468207TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGGATGC23550.1426904326519335TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGTTGC23080.13984268304062103TruSeq Adapter, Index 8 (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)