FastQCFastQC Report
Tue 26 Nov 2024
HHNHYAFX7_n01_13013in.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHNHYAFX7_n01_13013in.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5384967
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2441804.5344753273325535TruSeq Adapter, Index 1 (100% over 50bp)
CCTAGGGCCTCGCAGACCCATCACGGGTCCAGATCCCGGTCTTTTCGCAG100540.18670495102384102No Hit
CCTCTGCAGTCGAGCCAGAACCCTGTTTGAATTCACTTCCGGCGTGAATG95900.17808837082938486No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)