FastQCFastQC Report
Mon 21 Aug 2023
HHMVCDRX3_n02_S4_D28_PBMC_BCell.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHMVCDRX3_n02_S4_D28_PBMC_BCell.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83539344
Sequences flagged as poor quality0
Sequence length90
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT6429310.7696146141631182No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT4794850.5739630897748013No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2702570.3235086452199098Clontech SMART CDS Primer II A (100% over 26bp)
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT1611410.1928923454318722No Hit
CTTCTGTTGGTACCAATAGACATATTGTTTTGGCAGTGTATCTCCAGAGC1206630.14443852946702573No Hit
GGTGATCCTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTGGCTGTG1194640.14300327759336964No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC1173760.14050385648228217No Hit
CTCCAGAGCAGGTGATCCTGGCCGTCTGTCCTGGGGCCACTGACACCGAG1150650.13773749528126533No Hit
GTTTTGGCAGTGTATCTCCAGAGCAGGTGATCCTGGCCGTCTGTCCTGGG1067230.1277517812445355No Hit
GGCGTCTGGCCTGACTTCTGTTGGTACCAATAGACATATTGTTTTGGCAG1060600.12695814321931953No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA1059110.12677978414577926No Hit
GCTCAGGGATCCCTGAGGGCCTCTCAGTGTCCTTATATATAACCATTACA1020110.12211132517392044No Hit
GCCTGACTTCTGTTGGTACCAATAGACATATTGTTTTGGCAGTGTATCTC988130.11828318881699622No Hit
GGTACCAATAGACATATTGTTTTGGCAGTGTATCTCCAGAGCAGGTGATC976990.11694968540811142No Hit
CACCGAGGGTGGCTGTGTCAGTTCATAGGAGGCCTCAGAGCCTGTGCAGA976850.1169329268374432No Hit
CTCAGGGATCCCTGAGGGCCTCTCAGTGTCCTTATATATAACCATTACAG973160.11649121879625964No Hit
CAGAAAATCGCTCAGGGATCCCTGAGGGCCTCTCAGTGTCCTTATATATA960870.1150200556997431No Hit
GGCCTGACTTCTGTTGGTACCAATAGACATATTGTTTTGGCAGTGTATCT959930.11490753386811368No Hit
GCTGTGTCAGTTCATAGGAGGCCTCAGAGCCTGTGCAGAGAGTGAGGAGG957230.1145843328623696No Hit
GCAGTGTATCTCCAGAGCAGGTGATCCTGGCCGTCTGTCCTGGGGCCACT956620.1145113133758867No Hit
GTTGGTACCAATAGACATATTGTTTTGGCAGTGTATCTCCAGAGCAGGTG954700.1142814815495798No Hit
CCTGACTTCTGTTGGTACCAATAGACATATTGTTTTGGCAGTGTATCTCC923420.11053713804599662No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG909550.10887684250908171No Hit
GGCAGAAAATCGCTCAGGGATCCCTGAGGGCCTCTCAGTGTCCTTATATA889120.10643128823228488No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC889070.10642530302847483No Hit
CTTATATATAACCATTACAGGCGTCTGGCCTGACTTCTGTTGGTACCAAT887430.10622898834350436No Hit
CTCTTGTCCCTGAGGTGGAGGCAGAAAATCGCTCAGGGATCCCTGAGGGC858450.10275996421518464No Hit
GTCAGTTCATAGGAGGCCTCAGAGCCTGTGCAGAGAGTGAGGAGGGGGAG845320.10118824969465884No Hit
GACATATTGTTTTGGCAGTGTATCTCCAGAGCAGGTGATCCTGGCCGTCT835870.1000570461745546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)