FastQCFastQC Report
Mon 21 Aug 2023
HHMVCDRX3_n02_S3_D7_PBMC_BCell.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHMVCDRX3_n02_S3_D7_PBMC_BCell.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123596032
Sequences flagged as poor quality0
Sequence length90
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT7244600.5861515036340326No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5443200.44040248800220383Clontech SMART CDS Primer II A (100% over 26bp)
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA2571840.20808435015130583No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT1914750.15492002202789162No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC1460610.11817612397135857No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)