FastQCFastQC Report
Mon 21 Aug 2023
HHMVCDRX3_n02_S2_D3_PBMC_BCell.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHMVCDRX3_n02_S2_D3_PBMC_BCell.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127265777
Sequences flagged as poor quality0
Sequence length90
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT6368310.5003945404741449No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA3379900.2655780744575189No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2898450.22774779428722616Clontech SMART CDS Primer II A (100% over 26bp)
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC2338340.18373674801828302No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT2256520.17730768264590094No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)