FastQCFastQC Report
Mon 21 Aug 2023
HHMVCDRX3_n01_S4_D28_PBMC_TCell.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHMVCDRX3_n01_S4_D28_PBMC_TCell.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81526002
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATATAAATGCGTAGGGGTTTT1618040.19846919514095637No Hit
TGTTCGTTATCGGAATTAACCAGACA1398020.17148148636063376No Hit
GCACCTGCGGCGGCTCCACCCGGGCC1371010.16816843293750625No Hit
ACCTTTAAGTTTCAGCTTTGCAACCA1150050.14106542351972565No Hit
AGTTGGTTGATTGTAGATATTGGGCT943490.11572872173959911No Hit
GCTGCGGATATGGGTACGGCCCGGCG817900.10032382061369818No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)